Contract Research Services

BT and C, Inc. has served as a CRO since 1989 in a wide variety of research projects. The experience gained through these projects has endowed us with a broad base of experience in genetic engineering and tissue culture, especially as it relates to product development. We use our knowledge of science and our understanding of existing technologies to solve problems faced by scientists and administrators alike. General examples of contract research conducted at BT and C are below, followed by specific Project Titles. For details on our experience see our background information.

Development and optimization of genetic engineering and molecular biology processes:

Nucleic acid isolation and clean-up for application to HTS
Development of novel DNA labeling methods
Development of PCR ELISA methods for HTS
Design and optimization of PCR ELISAs for diagnostic tests

Generation of novel cell lines and methods for cell-based assays:

Application of Caco-2 cells to HTS screening
Immortalization of tissue culture cells for drug screening
Development of keratinocyte growth assays
Development of methods to reduce variation in 96-well cell-based assays

Project Titles

Application of lytic enzyme preparation to the protoplast transformation of yeast

Assay development for the detection of apoE genes (Alzheimer’s disease)

Assay development for the detection of GMO in food products using the polymerase chain reaction

Assessment of a bead-based polymerase chain reaction detection system

Assessment of keratinocyte tissue culture system for skin regeneration product

Design and development of antigen labeling system for the detection of multiple nucleic acid sequences

Design, development, and commercialization of an aseptic microtiter plate and vial freeze-drying device

Development of a PCR-ELISA for the measurement of cDNA

Development of an incubator to reduce the edge effect and variation in cell-based assays

Development of cryopreservation methods for the storage of cells for cell-based assays

Development of EPA approved water quality proficiency testing kit

Development of high-throughput plasmid purification system used for genetic engineering

Development of methods for high-throughput mechanical disruption of fungi and plant materials

Development of microbial quality control standards

Development of PCR-ELISA assay for HLA tissue typing

Development of PCR-ELISA for the detection of beta-thalassemia

Development of QC assay for the validation of Caco-2 cells

Effect of topical antibiotics on porcine keratinocyte viability as measured by MTS and LDH

Enzymatic analysis of lytic fermentation product

Evaluation of chemiluminescent systems for Western blot imaging

Evaluation of conditions for optimal culturing of mammalian cells

Evaluation of cytophobic surfaces for use in cell-based assays

Evaluation of non-binding surfaces on protease activities in vitro

Evaluation of non-binding surfaces on the stability of proteins

Expansion of polymerase chain reaction based HLA tissue typing kit

Genetic engineering for peptide gene construction for tissue engineering

Immortalization of cftr human lung epithelial cells using genetic engineering for HTS drug screening

Keratinocytes viability and growth assay development

Methods development for an isothermal nucleic acid amplification system

Methods development for new electrophoretic analytical tool

Process troubleshooting for microbial contamination

Protocol development for the use of surface activated microtiter plates for PCR-ELISAs

Proto-type DNA ELISA test development

Sample preparation methods development for high-throughput sequences

We have extensive experience in tissue culture.
Tissue Culture Research
Example: Keratinocytes growing from a skin explant

PCR Assay Development
Example: Detection of GMO sequences in soy

Molecular Biology Research
Example: Synthesis of recombinant proteins in yeast.